Haplotype sharing refines the location of an imprinted quantitative trait locus with major effect on muscle mass to a 250-kb chromosome segment containing the porcine IGF2 gene.
نویسندگان
چکیده
We herein describe the fine mapping of an imprinted QTL with major effect on muscle mass that was previously assigned to distal SSC2p in the pig. The proposed approach exploits linkage disequilibrium in combination with QTL genotyping by marker-assisted segregation analysis. By identifying a haplotype shared by all "Q" chromosomes, we map the QTL to an approximately 250-kb chromosome segment containing INS and IGF2 as the only known paternally expressed genes. This considerably reinforces the candidacy of these genes, justifying their detailed analysis.
منابع مشابه
Characterizing a distal muscle enhancer in the mouse Igf2 locus.
Insulin-like growth factor-2 (IGF2) is highly expressed in skeletal muscle and was identified as a quantitative trait locus for muscle mass. Yet little is known about mechanisms of its regulation in muscle. Recently, a DNA segment found ∼100 kb from the Igf2 gene was identified as a possible muscle transcriptional control element. Here we have developed an in vivo reporter system to assess this...
متن کاملIGF2 : The Use of a Paternally Expressed QTL influencing Muscle Mass in Marker Assisted Selection in Commercial Pig Populations
Recently a Quantitative Trait Locus (QTL) with major effect on muscle mass and fat deposition was mapped to the IGF2 locus on the distal end of Chromosome 2 in pigs (SSC2). This QTL was found in experimental crosses of Pietrain and Large White on the one hand (Nezer et al., 1999) and of Wild boar and Large White on the other (Jeon et. al., 1999) and explained 15 – 30 % of the variation in muscl...
متن کاملNucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene.
The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5' flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, ...
متن کاملLong-range chromatin interactions at the mouse Igf2/H19 locus reveal a novel paternally expressed long non-coding RNA
Parental genomic imprinting at the Igf2/H19 locus is controlled by a methylation-sensitive CTCF insulator that prevents the access of downstream enhancers to the Igf2 gene on the maternal chromosome. However, on the paternal chromosome, it remains unclear whether long-range interactions with the enhancers are restricted to the Igf2 promoters or whether they encompass the entire gene body. Here,...
متن کاملI-50: Embryo Loss Due to Epigenetic Anomaliesin the Male Germ Line: Role of Estrogen
Background: To investigate if aberrant methylation and expression of imprinted genes of the Igf2-H19 locus in the spermatozoa and embryos could be a paternal epigenetic factor involved in early embryo loss To elucidate the role of estrogen in acquisition of the imprinting at the Igf2-H19 locus during spermatogenesis Materials and Methods: Adult male rats of Holtzman strain were administered tam...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Genetics
دوره 165 1 شماره
صفحات -
تاریخ انتشار 2003